| To research the viable time and the influencing factors of Plasmodiophora Brassicae resting spores in different temperatures of water environment. Detection of dormant spores of Plasmodiophora Brassicae by fluorescence quantitative PCR in water at 16-25℃. The effects of different water temperature COD DO and Conductivity on the resting spores of P.Brassicae were analyzed. The experimental results show that at 25℃ P.Brassicae most slowly, which among 16℃, 19℃, 22℃ and 25℃. At 35day the COD, DO and conductivity were 86.2 mg/L, 5.95 mg/L and 103 S/cm respectively on the 35th day, and there were significant differences with other temperatures. In conclusion, the survival ability of P.Brassicae was stronger than that of other temperatures at 25℃. |
