| This study aimed to obtain actomycetes with high activity against plant pathogens from soil. Soil
samples were collected from woodlands of Lake Chi’ao in Shenzhen. Actinomycetes strain A50 was isolated using
serial dilution and plating method on Gause's Synthetic Agar Medium. Based on morphological, physiological and
biochemical characteristics, as well as the analysis of 16S rDNA, the strain A50 was was identified as Streptomyceyes
nodosus. The antifungal activity of A50 was studied by flat confrontation. The result of mycelium growth rate showed
that A50 had a broad spectrum inhibitory activity to all the tested 5 plant pathogenic fungi. Among these, activity
against Bipolaris maydis was the highest with the width of inhibitory zone above1.5cm. The fermentation solution of
A50 displayed 68% inhibition activity to sclerotium treated after 72h. A50 had big potential and wide prospect for
controlling plant pathogens. |
