| Camellia nitidissima is rich in flavonoids. As an important natural product, flavonoids possess a variety
of biological functions, such asoxidation resistance, blood sugar lowering, anti-cancer, anti-tumor and other effects.
Among these functions, oxidation resistance is most prominent one and forms the basis of other functions. In order to
realize the efficient extraction, separation and determination of flavonoids in C. nitidissima and to develop C. nitidissima
flavonoids product, this paper analyzed the flavonoids and the flavonoid constituent in C. nitidissima, introduced various
biological functions of flavonoids and conducted the summary and research of the extraction (water extraction, organic
solvent extraction, ultrasonic assisted extraction and enzymatic hydrolysis), separation (macroporous resin adsorption,
chromatography and membrane separation) and determination (spectrophotometry, high performance liquid chromatography, liquid chromatography and mass spectrometry) methods of flavonoids in C. nitidissima. At present, the major extraction method
of flavonoids in C. nitidissima is the organic solvent extraction method which combined with ultrasonic method, may achieve
better extraction effect; the separation of flavonoids in C. nitidissima were mostly conducted by macroporous resin adsorption
method and chromatography; spectrophotometry is the major method used for the determination of flavonoids in C. nitidissima
while high performance liquid chromatography and liquid chromatography-mass spectrometry have great application potential,
which would provide theoretical support for development and utilization of the C. nitidissima. |
