| 【Objective】The research aimed to study the optimal hormone concentration for the rapid propagation
of caladium bicolor tissue, and to provide reference and help for its factory production.【Method】Young leaves
developing from C. hortulanum ‘Wightii’ and C. hortulanum ‘Postman Joyner’ were used as the explant to make
an optimization experiment of callus induction and callus proliferation differentiation system, by taking tissue culture
and propagation in different formulations of 6-BA and NAA on MS basal medium. 【Result】Different combinations
of plant growth regulators showed different effects on callus induction, differentiation and proliferation within a certain
mass concentration. All the C. bicolors could induce callus and regenerated plants in these different formulations. After
transplanting to the matrix mixed with peat soil, vermiculite and perlite, the survival rate of seedling was more than 90%.
【Conclusion】The results showed that MS+6-BA 4.0 mg/L+NAA 0.5 mg/L was the best culture medium for callus
induction of C. bicolor with short start-up time and high cure rate, while MS+6-BA 2.0 mg/L+NAA 0.2 mg/L was most
effective for differentiation and proliferation with the highest callus differentiation rate and the best growth of test-tube
seedlings. |
