| 【Objective】A wild strain of suspected Tricholoma giganteum collected in Shaoguan University,
Northern Guangdong Province was identified and the suitable mycelium culture medium was screened, which provided basis
and reference for the enrichment of its biological resources and the development and utilization of the strain. 【Method】
Using the wild strain of the suspected T. giganteum as tested material, the pure mycelium was isolated and purified by tissue
isolation from the wild strain fruiting body. Identification was carried out by the morphological characteristics analysis of
fruiting bodies, biological characteristics analysis of mycelium, and ITS sequence cloning and analysis. In order to screen the
most suitable mycelium culture medium, mycelium culture experiment was carried out in PSA medium, yeast extract medium
and cottonseed shell medium, respectively.【Result】The cloning and analysis of ITS sequence showed that the suspected
wild T. giganteum had high similarity with the T. giganteum that reported in GenBank and Ident was between 82.23% and
90.06%, The sequence showed highest similarity with sequence JX041888.1 and its Ident was 90.06%. Combined with traditional morphological analysis, the strain was identified as wild T. giganteum and named as Tsg1. The mycelium could
be cultured in PSA medium, yeast extract medium and cottonseed shell medium. But the mycelium was the densest and the
strongest in the cottonseed shell medium, with the growth rate of 3.68 mm/d.【Conclusion】This strain is a wild T. giganteum,
and cottonseed shell medium is the most suitable medium for mycelium culture. |
