| Agrobacterium tumefaciens-mediated transformation (ATMT) is one of the most revolutionary techniques
in fungal research in recent 20 years. As a transformation tool, it has been widely used in the study of fungal gene
function, which made people have a better understanding of fungal genomics. Combined with phenotype screening of
mutants, random T-DNA obtained by ATMT was inserted into mutant library and the genetic basis of many processes
was elucidated. ATMT possesses the advantages of simple operation, wide transformation receptors, high transformation
efficiency and high stability of transformants etc.Magnaporthe oryzae, one of the top ten important fungi in plants, is
the pathogenic fungus that causes rice blast. At present, the most economical and environmentally-friendly method to
control rice blast is to cultivate rice resistant varieties. It is of great theoretical and practical significance to explain
the pathogenic mechanism and physiological characteristics and cultivate disease-resistant varieties by cloning the pathogenic genes related to Magnaporthe oryzae and studying their functions. ATMT provided an important tool for the study
of insertion mutation, gene knockout and marker cloning of Magnaporthe oryzae. The principle, characteristics, steps, and
influencing factors of ATMT and its application in functional genomics study of Magnaporthe oryzae were reviewed in order
to provide relevant references for the study of genetic transformation of fungi such as Magnaporthe oryzae etc. |
