| 【Objective】The embryonic development process of the Epinephelus coioides and the expression of
Dmrts gene in this process were studied to provide a basis for further study on the function of Dmrts gene in E. coioides.
【Method】The fertilized eggs obtained by natural spawning method were incubated in a constant temperature (24±0.5℃ )
and in still water, and cultured in seawater with a salinity of 31.0. The whole embryonic development process was
completed in about 29 h 50 min and entered the post-embryonic development stage. The embryonic development process of the E. coioides was observed by light microscopy. The embryos in the cleavage stage, blastocyst stage, gastrula
stage, neurula stage, organogenesis stage and newly hatched larvae stage were stored in RNAlater with 5 parallels in each
group. 【Result】The relative expression of Dmrt in different embryonic development stages were analyzed by fluorescence
quantitative analysis. The results showed that the Dmrt gene of the E. coioides began to express from the cleavage stage
during embryonic development. The expression level of Ecdmrt1 (E. coioides Dmrt1) reached the peak at the blastocyst stage,
and the expression level of Ecdmrt1 gene was relatively low at the cleavage stage and larvae stage. The expression level of
Ecdmrt1 gene at the blastocyst stage was 5.4 times that of cleavage stage. The relative expression of Ecdmrt2 (E. coioides
Dmrt2) gene was relatively stable at the early embryonic stage but increased significantly at the neurula stage (P<0.05),
which was 9.2 times that of cleavage stage. The Ecdmrt2 gene had the highest expression level in the newly hatched larvae,
which was 17.6 times that of the cleavage stage. The expression level of Ecdmrt2b gene increased gradually during embryonic
development and reached the peak at the organogenesis stage (P<0.05) while the expression level decreased at the newly
hatched larvae stage. The expression level of Ecdmrt3 (E. coioides Dmrt3) was relatively low in the early stage of embryonic
development, and significantly increased at the organogenesis stage (P<0.05), which was 7.1 times that of the cleavage stage,
and reached the peak at the newly hatched larvae stage. 【Conclusion】Ecdmrt gene expressed from the cleavage stage
during embryonic development, and may be involved in the development of embryonic organs and larvae. |
