| 【Objective】 To establish a method for the determination of three phenolic acid compounds (vanillic acid,
p-hydroxybenzoic acid and ferulic acid) in potato soil in a rapid and accurate manner，a study on the influence of different
continuous cropping patterns (common continuous cropping, corn/potato rotation and intensified continuous cropping) on
three phenolic acid compounds in potato soil was conducted. 【Method】 The wavelength, mobile phase system, column
temperature and other parameters were optimized in chromatographic condition by means of high performance liquid chromatography (HPLC)，and the phenolic acids in potato soil under different continuous cropping patterns were determined in
the optimized chromatographic condition. 【Result】 In certain chromatographic condition (Agilent XDB-C18 column: 250×4.6
mm, 5 μm; detection wavelength: λ=280 nm; column temperature:35℃ ; injection volume: 20μL; mobile phase: 75%A liquid
(glacial acetic acid regulation, pH=2.60); 25% B solution (acetonitrile) isocratic elution; dual-pump volume flow rate: SI=0.8
mL/min). there was a good linear relationship between the three phenolic acids (within a reasonable concentration range) and
peak area with the correlation coefficients greater than 0.99, the recovery rates between 94.37% and 100.46%, and the relative
standard deviations of reproducibility experiments below 0.18. The content of the three phenolic acids in potato soil was detected
under different continuous cropping patterns. There was no significant difference among the content of the three phenolic acids
under the blank control before and after the growth period. The content of the three phenolic acids under the three continuous
cropping patterns in the mature stage was lower than that in the early stage. The content of phenolic acid in corn-potato rotation
soil in the mature stage was lower than that in the other two continuous cropping patterns, with significant difference from the
blank control. 【Conclusion】 With the features of simple, accurate and reliable, optimized HPLC can determine the content
of the three phenolic acids in potato soil effectively. The rotation of corn-potato can reduce the content of these three phenolic
acids in soil and alleviate the continuous cropping disorder caused by phenolic acids. |
