| 【Objective】Nannochloropsis oculata is a kind of excellent single-celled diet microalgae, and widely
used in aquaculture.The culture medium of Nannochloropsis oculata was optimized in order to improve its growth rate and
reduce the production costs. 【Method】Taking Ningbo University 3# Formula as the basic microalgae culture medium,
with CHCOONa as carbon source, KNO3, (NH2)2CO and NH4CL as nitrogen source, KH2PO4 and NaH2PO4 as phosphorus
source, FeSO4 and FeC6H5O7 as iron source, the effects of different nutrient sources such as carbon, nitrogen, phosphorus,
iron,Vitamin B1 and Vitamin B12 on the growth and propagation of N. oculata were analyzed by one-factor experiment
and orthogonal test. 【Result】The optimized culture medium based on nature sea water was formulated as follows: 3 g/
L CHCOONa, 20 mg/L NH4Cl-N, 2 mg/L KH2PO4-P, 3 mg/L FeSO4-Fe, 0.05 mg/L Vitamin B1 and 0.005 mg/L Vitamin
B12. The N.oculata was cultured on optimized medium and Ningbo University 3# medium and the results showed that the
biomass (cell density) of N. oculata cultured on optimized medium for 2-6 days was 2.21, 2.55, 2.30, 1.97 and 1.7 times higher than that of Ningbo University 3# Medium respectively. By the end of the sixth day, the biomass (cell density) of N.
oculata on the optimized medium was up to 1.74×107 cell/mL, which was 1.7 times higher than that on Ningbo University
3# medium. 【Conclusion】The optimized medium extrenely enhamced the biomass of N. oculata and it was a good medium
for N. oculata. |
