| Loop-mediated isothermal amplification (LAMP) technology has attracted many researchers’attention for
its fast, sensitive and low-consumption characteristics since it was developed in 2000. This method has been widely studied
and applied in the detection and monitoring of microorganisms related to human, animals, plants and environment. Its
detection sensitivity is generally higher than that of traditional PCR. However, the traditional LAMP is time-consuming and
easy to cause pollution in the detection of products, and has disadvantages of inconvenient interpretation in the field or point
of care. This paper summarized, illustrated and discussed the methods of LAMP technology combining with other product
detection technologies. Visual LAMP has the characteristics of low cost and being suitable for multi-environment. Real-time,
micro-fluidic and electrochemical LAMP can effectively improve the accuracy of sample detection and reduce detection
time. Lateral flow detect LAMP has the characteristics of strong operability and easy interpretation. According to the current
research progress of LAMP technologies and the analysis of their advantages and disadvantages, it is predicted that the future
development direction and focus of LAMP technology may be the development of hand-held portable detectors. |
