| 【Objective】 The pathogen of blistering disease with nasal and coronet in two swine farms in Guangdong
province was detected, and new strains of Senecavirus A (SVA) were obtained, which provided materials for subsequent
research. 【Method】 RT-PCR and real-time RT-PCR were used to screen the clinical samples. The clinical samples
were isolated, treated and cultured with ST cells and BHK-21 cells. The virus was purified by sucrose density gradient centrifugation method and observed by electron microscopy. The specific primers of SVA were designed to amplify the cell
supernatant, and sequenced for sequence analysis. The TCID50 of the virus isolated was determined. 【Results】 The swinery
were diagnosed as SVA by RT-PCR and real-time RT-PCR. It was found that the cultured cells had CPE from the 6th
generation. The virion observed by electron microscopy was about 25 nm. The amplified sequences were submitted to the NCBI
Blastn, which were identified as SVA. The TCID50 result of the strain SVA CH-GDBL1-2016 was 106.8 and the TCID50 result of
the strain SVA CH-GDBL2-2016 was 106.7.【Conclusion】 The strains SVA CH-GDBL1-2016 and SVA CH-GDBL2-2016
provide a material basis for the subsequent diagnosis and vaccine development of Senecavirus disease. |
