|
| Development and Application of Molecular Markers for Radish Leaf Edge Cracking Traits |
| |
| DOI：10.16768/j.issn.1004-874X.2019.07.003 |
|
| Hits: 1913 |
| Download times: 706 |
| Abstract: |
| 【Objective】The study identifies the genetic characteristics of radish leaf cracking traits and develop a
molecular marker which linked to lobed leaf traits.【Method】The radish variety “WUQING No.1”(as female parent) and
“XINZOUHUAYE” (as the male parent) were used to make the hybrid F1，then self-bred and backcross of F1 were made
to get F2 and BC1. And the genetic characteristics of lobed leaf traits were explored.【Result】The results showed that the
leaf edge of F1 was completely lobed. The separation ratio of leaf edge lobed to non-lobed plants in BC1 and F2 were 1∶1
and 3∶1，indicating that the phenotypic characteristic of radish leaf edge lobed matched with dominant genetic heredity
pattern and was controlled by a single genetic locus.The BSA and AFLP were used to screen the molecular markers which
were closely linked to the lobed leaf edge gene of radish，and the SCAR marker primer HY-18 was closely linked with the
lobed leaf edge gene of radish，with the genetic distance of 3.32 cM.【Conclusion】The obtained markers lay a theoretical
foundation for molecular marker assisted breeding and the cloning of lobed leaf genes. |
|
View Full Text
View/Add Comment Download reader |
