| 【Objective】 The study was to develope a rapid detection method for veterinary drug residues in
whole egg, albumen, and yolk. 【Method】 Taking sulfamethazine as a case, a method was developed for rapid detection
of sulfamethazine in whole eggs, yolks, and albumens. The sample pretreatment was conducted by using liquid-liquid
extraction to achieve the extraction and purification of target compounds. And the target compounds were detected by high
performance liquid chromatography-electrospray ionization tandem mass spectrometry that with good selectivity and high
sensitivity. 【Result】 As verified by spike matrix test, the method was proved to be stable and reliable. The recoveries of
sulfamethazine in whole eggs, yolks, and albumens were in range of 78.4%-85.2%, 77.9%-89.0% and 83.0% - 88.1%,
respectively. The detection limit of the method was 0.5 ng/g for the target compounds in whole eggs, yolks, and albumens. Finally, the developed method was applied in sulfamethazine analysis of 15 egg samples from Guangzhou City. The results
showed that sulfamethazine was detected in only one sample, and the contents of sulfamethazine in whole eggs, yolks, and
albumens were 0.66, 0.94, and 0.47 ng/g, respectively. 【Conclusion】 Due to no column chromatography procedure in sample
pre-treatment, the method has the advantages of simple operation, low cost and high sensitivity. In addition, this method has
good recovery and stability, which is suitable for rapid detection of sulfadimidine in large quantities of whole egg, albumen, and
yolk samples. |
