| 【Objective】Fresh-cut Pueraria (southern medicinal plant) is prone to browning, the effect of nitric oxide
(NO) treatment on the inhibition of browning of fresh-cut Pueraria at was studied in this paper.【Method】Fresh-cut
Puerariawere dipped into 0.5, 1.0 and 2.0 mmol/L sodium nitroprusside (SNP) solution respectively for 15 min and then
were drained and sealed with polyethylene and stored at constant temperature . And the physiological and biochemical
indexes related to browning were determined regularly.【Result】The results showed that, compared with the control group,
the browning of fresh-cut Pueraria was inhibited by being treated with different concentrations of sodium nitroprusside.The
browning index of treatment with 2.0 mmol/L sodium nitroprusside was lowest, which was only 62.4% of the control at 10 d.
Based on the determination of the physiological and and biochemical indexes related to browning, different concentrations of
SNP treatment could inhibit the activity of PAL after 6 d, decrease the total phenol content, and activity of PPO and promote the content of total flavonoid during the whole storage, and the above indexes were inhibited or promoted significantly with
the increase of the concentration of SNP. The contents of total phenol and flavonoid of the treatment with 2.0 mmol/L SNP
were 66.4% and 542% of the control at 10d, while the activity of POD and the content of malondialdehyde were decreased
significantly by the treatment of 2.0 mmol/L SNP during the whole storage, which could inhibit the brownin go ffresh-cut
Pueraria. According the correlation analysis on the physiological indexes of fresh-cut Pueraria treated with 2.0 mmol/L SNP,
the browning degree was positively correlated with PAL activity, total phenol contentand flavonoid content and negatively
correlated with the activity of POD and PPO, however, it was not correlated with MDA significantly.【Conclusion】2 mmol/L
SNP treatment could effectively inhibit the browning of fresh-cut Pueraria and improve its functional quality, providing reference
to the color protecting of fresh-cut Pueraria. |
