| 【Objective】The egg production of Hermetia illucens L.is greatly affected by continuous low temperature
or rainy weather. It is helpful to standardize the production of H. illucens by refrigerating the surplus eggs during the peak
spawning period.【Method】In this paper, the egg hatching rate of H. illucens at different developmental stages after being
refrigerated at 12℃ for different days, and the hatching, growth and development of eggs at gastrula stage at 32℃ after
being refrigerated at 12℃ for 5, 10 and 15 days respectively was studied. 【Result】The results showed that the primiparous
eggs could not be refrigerated directly. Refrigerated at 12℃ for 1 day and then incubated at 32℃ , the mortality rate of
eggs was 100%. The eggs at blastoderm and germ band formation stage, gastrula stage, dorsal closure stage and hatching
stage were refrigerated at 12℃ and then incubated at 32℃ . The average hatching rate were 94%, 94%, 27% and 0%,
respectively. Therefore, the eggs at dorsal closure stage and hatching stage were not suitable for cold storage. The hatching
rate of eggs during blastoderm and germ band formation stage was reduced to less than 80% after 13 days of cold storage.
The hatching rate of the eggs during gastrula stage was reduced to less than 80% after 15 days of cold storage. There were
no significant difference in the growth and development between the larvae hatched from the eggs at gastrula stage that
were refrigerated for 5 and 10 days and the larvae of control group. The survival rate of the eggs at gastrula stage which were refrigerated for 15 days was lower than that of the control group. There were no significant difference in the weight of
one hundred larvae. 【Conclusion】The eggs at gastrula stage refrigerated at 12℃ can maintain a high survival rate, and the
larvae can grow and develop normally. The results can be used to provide theoretical basis and practical guidance for industrial
production of H. illucens. |
