| 【Objective】In this study, the inheritance of the trait of white-partridge feathers in Wenchang chicken
was studied and the related genetic variation was identified., which provided references for the utilization of the whitepartridge population and molecular marker-assisted selection of white-partridge feather trait. 【Method】Firstly, the
inheritance of white-partridge feather trait was studied by reciprocal test. Then, SLC45A2 was selected as a candidate gene
based on the genetic characteristics of white-partridge feather and results of previous researches. The variation in the exon
domain of SLC45A2 gene was analyzed by using re-sequencing method. Lastly, the relationship between missense mutation/ nonsense mutation in the exon domain and the trait of white-partridge feather was verified. 【Result】 The results of reciprocal
test showed that the trait of white-partridge feather was recessive inheritance compared with that of yellow-partridge feather.
The amplification results indicated that there were five SNPs in SLC45A2 gene, in which three were located in exon domain,
including two were missense mutation (c.a74g and c.g1154c) and one was synonymous mutation (c.c561t). Finally, the result
showed that C.G1154C site of SLC45A2 gene was completely linked with the white-partridge feather trait based on missense
mutation study in Wenchang chicken, Bawang chicken, Qingyuan partridge chicken, Xinghua chicken and Gallus gallus.
【Conclusion】 The trait of white-partridge feathers showed recessive inheritance and c.g1154c site might be the causation. |
