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Study on the Effect of Trifolium pratense L. as an Indicator Plant of Sr and Cs Pollution |
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DOI:10.16768/j.issn.1004-874X.2020.02.012 |
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Abstract: |
【Objective】Trifolium pratense L. was used as tested material to explore the sensitivity and
physiological characteristics to Sr and Cs through pot hydroponic experiment.【Method】Three treatments, Sr, Cs
and Sr+Cs, were set up in the experiment. 5 concentration gradients were set in each treatment, including 0 mg/L,
0.1 mg/L, 0.5 mg/L, 1 mg/L and 2 mg/L. Under the treatments of different concentrations, the growth indexes such
as canopy diameter, leaf area per plant and biomass of clover plants, physiological and biochemical indexes such
as chlorophyll content and MDA content in leaves, and the absorption of Sr and Cs in above-ground and underground parts of clover were measured. In this paper, the sensitivity of clover to Sr and Cs was studied by comparingthe differences of various indexes among different treatments.【Result】Under the treatment of Sr, leaf area per plant of clover
increased significantly, while MDA content in leaves increased gradually with the increase of treatment concentration. When treated
with 1.0 mg/L Sr, MDA content was significantly higher than that of the control group. Under the treatments of Cs and Sr+Cs, the
growth and morphological indicators of clover plants, such as plant crown diameter, leaf area per plant, and above-ground biomass,
were severely inhibited, which decreased by 28.0%-35.0% and 29.5%-37.1%, 31.6%-41.7% and 16.8%-21.1%, 44.0%-48.0%
and 47.0%-56.0%, respectively. In addition, MDA content increased gradually with the increase of treatment concentration, and the
leaves of clover also tended to be yellow. At the same time, the amount of Cs absorbed by clover was much higher than that of Sr, and
Sr and Cs were mainly accumulated in the above-ground part.【Conclusion】The clover was sensitive to Cs, which showed a decrease
in chlorophyll content and an increase in MDA accumulation in leaves at physiological level, and a decrease in leaf area per plant and
biomass, inhibition in growth and etiolation in leaves at individual level. It had the potential to be an indicator plant of Cs pollution. |
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