|
| Research on Aniline-degrading Bacterium Rhodococcus sp. AN-P1 in Response to Aniline Based on Proteomics |
| |
| DOI：10.16768/j.issn.1004-874X.2020.04.012 |
|
| Hits: 1520 |
| Download times: 633 |
| Abstract: |
| 【Objectives】The response mechanisms of microorganisms under aniline stress were studied to reveal the internal molecular mechanism for Rhodococcus sp.AN-P1 to adapt to and degrade aniline, supplement the gap of anilinedegrading bacterium tolerance mechanism under aniline stresss, provide a good target for further regulation and improvement of the ability of microorganisms to degrade aniline, and lay a theoretical foundation for the research of on bioremediation.【Methods】Two-dimensional electrophoresis was used to separate and purify the proteome of Rhodococcus sp.AN-P1 expressed under the conditions of aniline and citric acid. Matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry（MALDI-TOF-MS）was used to identify the significantly differentially expressed proteins. Finally,the detailed information of protein spots was obtained from the NCBInr protein database.【Results】With aniline and citric acid as the sole carbon source, Rhodococcus sp. AN-P1 expressed 681 and 579 protein spots, respectively. 21 significantly different protein spots were selected for mass spectrometry analysis and related information of 17 protein spots was successfully obtained. These proteins were distributed in multiple metabolic systems such as signal transduction regulation, amino acid and energy metabolism, cell defense, and aniline degrading enzymes. It was found that the mass of most protein molecules was between 31 000 and 58 000, and the isoelectric point between 4 and 7.【Conclusions】Uunder the environment of aniline stress environment, Rhodococcus sp.AN-P1 could sense external stress by the signal transduction system under aniline stress environment, adjust the amino acid and energy metabolism system to resist the toxic effects of aniline, use the cell defense system to further improve its survival ability, and express aniline-degrading enzymes to degrade aniline for obtaining carbon source, nitrogen source and energy source. Ultimately, Rhodococcus sp. AN-P1 achieved the purpose of adapting to and degrading high concentration aniline. |
|
View Full Text
View/Add Comment Download reader |
|
|
|
