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| Analysis on CP Gene Sequence of Turnip Mosaic Virus Infecting Cabbage in Guizhou |
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| DOI：10.16768/j.issn.1004-874X.2020.04.014 |
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| Abstract: |
| 【Objective】The study was to identify the occurrence and distribution of cabbage Turnip mosaic virus（TuMV）in Guizhou Province, and provide guidance for scientific prevention and control.【Method】Samples of cabbage virus disease were collected in Anshun City, Weining County and Xiuwen County of Guizhou Province, and TuMV was detected by enzyme-linked immunosorbent assay（ELISA）and RT-PCR. The consistency of TuMV CP gene sequences was analyzed by relevant biological software, and the phylogenetic tree was constructed.【Result】Among 152 samples of cabbage virus disease, 66 samples were tested positive, with a detection rate of 43.42%. The nucleotide homology of 15 TuMV CP genes was 88.70%-100%, and it was found that 14 isolates belonged to basal BR and 1 isolate belonged to world-B group according to the phylogenetic tree construction of isolates in 6 TuMV groups.【Conclusion】TuMV is common in different cabbage planting areas of Guizhou Province and basal-BR is the dominant toxic source for the infection of cabbage. |
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