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| Optimization of High Frequency Embryo Induction Medium for Isolated Microspore Culture of Chinese Flowering Cabbage |
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| DOI：10.16768/j.issn.1004-874X.2020.05.005 |
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| Abstract: |
| 【Objective】The high frequency embryo induction medium for free microspore culture of Chinese flowering cabbage were optimized in order to improve embryo induction rate and create excellent DH lines for breeding practice.【Method】The NLN induction medium with different concentrations of sucrose and hormone（6-BA and NAA）were set, and 10 Chinese flowering cabbages with different genotypes were used as the experiment materials to conduct microspore culture and induction rate analysis for screening out the optimal induction medium.【Result】The results showed that a total of 7 genotypes produced microspore embryos, and significant differences in embryo induction rate were observed among different genotypes, indicating that genotype was the main factor affecting the formation of microspore. Sucrose concentration in the NLN induction medium had a great influence on the embryo production rate. In the hormone-free group, NLN（NLN-10）with 100 g/L sucrose had the highest microspore induction rate and the embryo production rates of the 7 genotypes were 3.45 - 30.46 embryos/dish. In the 1/2 NLN-10 culture medium treatment group, the embryo production rate was about 2/3 of that in the NLN-10 treatment group. In the NLN-13 treatment group, the embryo production rate was significantly reduced, with the highest rate of 14 embryos/dish. When the sucrose concentration reached 160 g/L（NLN-16）, the embryo production was inhibited. When adding 0.1 mg/L 6-BA and NAA to the NLN-10 medium respectively, the optimal embryogenesis could be obtained, with the highest embryo production rate of 46.93 embryos/dish created by “Rt19006”, which was 1.5 times higher than that of hormone-free group. When the concentration of 6-BA and NAA in NLN-10 reached 0.2 mg/L, the growth and development of embryos were inhibited.【Conclusion】An induction medium formula that can effectively promote the embryo production of microspore in Chinese flowering cabbage is screened out, that is: NLN culture medium containing 100 g/L sucrose and 0.1 mg/L 6-BA and NAA. |
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