|
| Isolation and Identification and Drug Susceptibility of Vibrio alginolyticus from Green Sea Turtle |
| |
| DOI：10.16768/j.issn.1004-874X.2020.06.014 |
|
| Hits: 1855 |
| Download times: 636 |
| Abstract: |
| 【Objective】Rotten skin is a common disease in hatchling breeding of green sea turtles（Chelonia mydas）, with high infectivity, morbidity and mortality. In order to determine the etiology and treatment of the disease, a pathogenic bacterial was isolated from the rotten tissue of flipper of diseased C. mydas.【Method】Suspected pathogens were isolated and obtained from limb lesions of diseased larval turtle and marked as CMRT91026. The morphological, physiological and biochemical characteristics as well as drug susceptibility were analysed. The phylogenetic tree was established on the basis of the identified 16S rRNA.【Result】The results showed that the isolated strain could grow on TCBS medium and the colony was yellow. The physiological and biochemical analysis indicated that the strain CMRT91026 was Gram-negative Brevi bacterium. The biochemical characteristics of the strain CMRT91026 on 1%NaCl phosphate glucose peptone water, sucrose, mannose and lysine decarboxylase media presented positive and it could grow in 3%-10% peptone. The homology of 16S rRNA among CMRT91026 and other typical Vibrio alginolyticus on NCBI was 100%. Thus, strain CMRT91026 was identified as V. alginolyticus. The antibiotic susceptibility test showed that strain CMRT91026 was sensitive to enrofloxacin, nalidixic acid, levofloxacin, minocycline, cefepime and ceftriaxone. It exhibited intermediate sensitivity to doxycycline, flurfenicol, tobramycin, spectinomycin, norfloxacin, aztreonam and resistance to 15 drugs such as neomycin, sulfamethoxazole compound, amoxicillin, gentamicin, amikacin and etc.【Conclusion】The strain is identified as V. alginolyticus and it is sensitive to quinolones（enrofloxacin）, tetracyclines（minocycline）and cephalosporins（ceftriaxone）. The test results contribute to the diagnosis and prevention of V. alginolyticus disease in larval green sea turtles. |
|
View Full Text
View/Add Comment Download reader |
|
|
|
