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| Analysis of Transcriptome and Exploration of Genes Related to Flavonoid Biosynthesis in Sauropus spatulifolius Beille |
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| DOI：10.16768/j.issn.1004-874X.2020.07.005 |
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| Abstract: |
| 【Objective】The study was conducted to obtain the transcriptomic characteristics and to identify the synthesis pathway genes related to flavonoid biosynthesis in Sauropus spatulifolius Beille.【Method】The high-throughput sequencing platform Illumina HiSeqTM 4000 was used to perform transcriptome sequencing and the unigene was assembled through de novo assembly by Trinity software. Then the functional annotation and metabolic pathway analysis of these unigenes were performed based on sequence homology.【Result】After quality control of sequencing data, a total of 88 396 692 highquality reads were obtained, and 46 600 unigenes were obtained through de novo assembly. The N50 length was 1 441 bp, with an average length of 877 bp. A total of 34 188（73.36%）unigenes were annotated in NR, SwissProt, KOG, GO and KEGG databases. Among them, 6 902 unigenes were annotated in the KEGG database, involving 132 metabolic pathways. It was identified that 56 unigenes were involved in flavonoid biosynthesis, 9 unigenes involved in flavone and flavonol biosynthesis, and 2 unigenes involved in isoflavonoid biosynthesis. 1 256 transcription factors（TFs）and 3 842 plant resistance genes（R genes）were also identified. MISA analysis showed that 46 600 unigenes contained 3 356 simple sequence repeats（SSRs）.【Conclusion】Transcriptome information characteristics of S. spatulifolius Beille were obtained by the high-throughput sequencing technology and biological information analysis, which provided a research basis for future researches to identify functional genes, flavonoid secondary metabolic pathways and their regulatory mechanisms. |
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