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| Occurrence and Subgroup Identification of Cucumber mosaic virus in Luoyang Area |
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| DOI：10.16768/j.issn.1004-874X.2020.07.014 |
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| Abstract: |
| 【Objective】The study was to identify the occurrence and subgroup types of Cucumber mosaic virus（CMV）on major vegetables in Luoyang City, and provide an important theoretical basis for the field diagnosis and prevention and monitoring to prevent the large-scale outbreak of CMV in this area.【Method】Samples with suspected virus symptoms collected from the Solanaceae, Cucurbitaceae and Leguminosae vegetables in Luoyang City, and were detected by RT-PCR with CMV primers, the obtained CMV CP gene sequences were analyzed by relevant biological software and the phylogenetic tree was constructed.【Result】Among 86 vegetable samples with suspected virus, CMV was detected in 13 samples from tomato, pepper, kidney bean and peanut, with a detection rate of 15.12%. The nucleotide homology of 4 CMV CP genes was 90.8%-98.8%, and it was found that the CMV isolates on these 4 crops belonged to CMV subgroup Ⅰ strains according to the phylogenetic tree construction of isolates in CMV subgroup Ⅰ and CMV subgroup Ⅱ .【Conclusion】CMV occurred in Solanaceae and Leguminosae vegetables in Luoyang area, and CMV Ⅰ may be the dominant virus, indicating that aphid prevention and control should be done in advance at the early stage of crop planting, and CMV Ⅰ disease resistance breeding should be actively carried out on relevant crops. |
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