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Effects of Nitrogen Sources on Manganese Toxicity in Sugarcane |
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DOI:10.16768/j.issn.1004-874X.2020.08.001 |
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Abstract: |
【Objective】The study was to explore the effects of nitrogen(N)sources on manganese(Mn)toxicity in sugarcane.【Method】Effects of N sources on leaf chlorosis, plant Mn absorption and distribution, and leaf photosynthetic and fluorescence characteristics in sugarcane seedlings were studied through the hydroponic tical.【Result】Under excessive Mn stress, Mn content in sugarcane seedlings increased significantly while leaf chlorophyll content (SPAD value)decreased significantly. Mn absorption of the plant and the seedling chlorosis affected by N sources were indicated as follows: the decrease in leaf SPAD value and seedling chlorosis were shown in the plants exposed to 0.5 mmol/L Mn solution with nitrate as the N source, while Mn had no significant effect on the plants with ammonium as the N source. There was no significant difference in Mn ratio in elder leaves to young leaves, Mn ratio in young leaves blade to the sheath and Mn distribution in leaf cell saps between the seedlings with different N sources after Mn treatment, however, Mn absorption and Mn contents of plants with ammonium source were significantly less than those of the plants with nitrate source. The differences in plant Mn absorption and content and the difference in leaf SPAD values between the plants with different N sources tended to increase with the increase of Mn treatment concentration(0, 0.1, 0.2 mmol/L)and Mn treatment time(0-21 d), respectively. Similarly, the plants with ammonium source had higher leaf photosynthetic rate, transpiration rate, stomatal conductance, actual photosynthetic efficiency, photochemical quenching coefficient and lower quantum yield of regulatory energy dissipation.【Conclusion】Mn-induced chlorosis in sugarcane seedings could be effectively alleviated by ammonium application. |
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