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| Optimization of Microspore Regeneration System in ggplant |
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| DOI：10.16768/j.issn.1004-874X.2020.08.003 |
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| Abstract: |
| 【Objective】The technology of microspores callus induction, growth and differentiation of eggplant （Solanum melongena） were studied to provide technical support for the establishment and application of the microspore regeneration system of eggplant.【Method】With the free microspores of diploid eggplant as materials, the effects of induction conditions,culture methods and culture medium types on the callus induction, growth and differentiation of eggplant microspores were explored, and the microspore culture system of eggplant was established.【Result】Under the dark static culture conditions, and replacing with some fresh culture medium for 2-3 times were beneficial to callus induction and growth. Upon the
differentiation culture of callus, the survival rate of callus was related to its size when the callus was directly transferred to solid medium. When the callus was more than 2 mm, the survival rate could reach 82.67%. The requirement of solid-liquid culture on callus size was not strict, adding 1-2 mL N3 liquid medium was conducive to the growth and survival of callus, while the survival rate of differentiation culture of callus would be reduced when the liquid medium was over 2 mL. The highest greening rate of callus（maturity）was 94.64% in MS solid medium with 36 g/L mannitol. The hormone combination of ZT 1.5 mg/L and NAA 0.05 mg/L was beneficial to bud differentiation, and the differentiation rate was 57.78%.【Conclusion】Under the dark still culture, changing part of medium regularly was beneficial to callus induction and growth. When 1-2 mL N3 liquid medium was added into solid medium, the survival rate of callus was the highest and the growth rate was fast. The hormone combination of ZT 1.5 mg/L + NAA 0.05 mg/L with 36 g/L mannitol on the solid medium was conducive to callus differentiation. |
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