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| Preliminary Study on the Mechanism of a Strain Bacillus licheniformis HS10 against Cucumber Downy Mildew |
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| DOI：10.16768/j.issn.1004-874X.2020.09.011 |
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| Abstract: |
| 【Objective】Bacillus licheniformis HS10 is a biocontrol strain obtained by a series of screening in the previous study. And the disease defense spetrum and mechanism of such strain were observed in this study.【Method】By comparing the pathogens with similar classification status with cucumber downy mildew, the litchi downy mildew, caused by Peronophythora litchii Chen ex Ko et al., was selected as control object. The litchi fruits after-harvest and in the field were pre-treated to observe the control effect of litchi downy mildew. A primary exploration on the disease defense mechanism was conducted by analyzing the crude protein produced by the strain HS10 and the plant disease-resistant enzyme activity.【Result】The HS10 strain had a control efficacy of 62.36% against post-harvest litchi downy mildew and a field control efficacy of 46.00%. The control efficacy of final concentration of 50 g/mL crude protein against cucumber downy mildew in the greenhouse was 60.08%. Extracellular crude protein could inhibit the growth of Phytophthora capsici, causing abnormal growth of the hypha tip, expansion and distortion. After the cucumber plants being treated with HS10 strain, PAL and POD increased in the early stage, then decreased, and increased rapidly in the late stage.【Conclusion】B. licheniformis HS10 also had control efficacy on other similar diseases. The strain inhibited the growth of hyphae through exocytic protein and affected relevant plant defense enzyme activity, which laid a foundation for further exploration of control mechanism. |
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