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| RNA Editing System Based on CRISPR/Cas13 and Its Application in Nucleic Acid Detection |
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| DOI：10.16768/j.issn.1004-874X.2020.11.027 |
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| Abstract: |
| Nucleic acid detection technology is widely used in agriculture, medicine, environment and other fields. The traditional nucleic acid detection technology has many limitations such as expensive equipment, complex operation and low sensitivity. Clustered regularly interspaced short palindromic repeats/CRISPR- associated gene 13（CRISPR/Cas13）is a novel CRISPR system that only targets RNA. It can specifically cleave single strand target RNA under the guidance of crRNA, and has the ability of nonspecific“collateral cleavage”. The nucleic acid detection technology based on CRISPR/Cas13 has the advantages of high sensitivity, strong specificity, convenient operation and low cost, which has broad application prospects. The classification and subtypes of CRISPR/Cas13 system and its component characteristics and molecular mechanism are reviewed, the generation and development of specific high-sensitivity enzymatic reporter unlocking （SHERLOCK）technology based on CRISPR/cas13 system is introduced, and its application in pathogen detection, drug resistance mutation detection, species identification and transgenic identification is summarized. At the same time, the existing problems based on CRISPR/Cas13 nucleic acid detection technology are pointed out, and the future application is prospected. |
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