| 【Objective】Quantitative real-time PCR（qRT-PCR）has become an main tool for gene expression analysis due to its high sensitivity and specificity, and selecting reference genes suitable for different conditions is the prerequisite for qRT-PCR analysis. The reference genes suitable for qRT-PCR analysis of different organs and petals in different periods of Camellia azalea were screened to provide usable reference genes for subsequent related gene function researches.【Method】In this study, six housekeeping genes were selected, which were the coding genes of transcription elongation factor（EF1α）, α-tubulin（TUA）, β-tubulin（TUB）, Ubiquitin（UBQ）, Actin and glyceraldehyde- 3-phosphate dehydrogenase（GAPDH）, and two different calculation programs GeNorm and NormFinder were used to evaluate the expression stability of 6 genes. The applicability of the selected reference genes were further verified by the expression mode of CaGASA3.【Result】In different organs, GeNorm and NormFinder were used to screen out the most stable genes. The top two were TUA and GAPDH. GAPDH had the best stability in the evaluation of the two calculation programs. In petals of different periods, the top 2 reference genes obtained by the two programs were TUB and UBQ. UBQ had the best stability in the evaluation of GeNorm program, and TUB had the best stability in evaluation of NormFinder program.【Conclusion】The optimal reference genes in different organs of C. azalea were TUA and GAPDH, and the most suitable reference genes in petals of different periods were TUB and UBQ. |
