| The study was conducted to analyze the genetic diversity of soybean resources in Guangdong Province, select effective SSR markers and construct DNA molecular ID for rapid identification.【Method】In this study, 30 pairs of SSR primers were used to amplify the genomic DNA of 96 soybean germplasm resources collected from 37 counties （cities）of 19 municipalities in Guangdong Province. The polymorphic results were obtained by sequencing, and could be used for cluster analysis and DNA molecular ID construction. Related variety traits were recorded and transformed to visual information.【Result】The results of capillary electrophoresis showed that 30 pairs of SSR primers had clear and stable polymorphic fragments among 96 soybean accessions. A total of 273 polymorphic alleles were amplified, and the average number of alleles per primer was 14.29. The range of polymorphism information content（PIC）revealed by different primers ranged from 0.3891 to 0.9310, the average polymorphism was 0.6786. Thirty pairs of primers could be used to distinguish soybean resources in Guangdong Province. Cluster analysis showed that the collected soybean samples could be divided into three groups with genetic diversity. Six pairs of primer combinations were selected according to the value of PIC to distinguish 96 soybean accessions from each other. 【Conclusion】Based on the amplification results of 6 pairs of SSR primers, the polymorphic fragments were sequenced and a set of molecular ID of 96 materials were successfully constructed through combined coding with numbers and English letters, providing an important basis for the identification of soybean resources in Guangdong Province. |
