| 【Objective】The induction of embryogenetic callus and proliferation of litchi leaves were studied in order
to lay a good foundation for the establishment and application of litchi regeneration system technology.【Method】With
the litchi leaves as materials, the influencing effects （genotype, source of materials, components of culture medium, etc.）
of litchi leaves on embryogenic callus induction were studied, and the proliferation experiment on subculture proliferation
of embryogenic callus was carried out.【Result】The sterile leaves germinating for about 2-4 weeks were cut into a size
of 1 cm2 with two cuts perpendicular to the main vein, and were inoculated facing down to the medium of MS+2,4-D 1.5
mg/L+KT 2.0 mg/L+sucrose 30 g/L+agar 7 g/L+PVP 500 mg/L+inositol 100 mg/L. After 2-3 subcultures, the varieties
of ‘Sanyuehong’and‘Yujinqiu’could induce embryogenic callus with better state and these callus could be used in
subsequent experiments, with the induction rates of 21.67 % and 42.50%, respectively. The subculture proliferation rate on
the 2,4-D 1.5 mg/L+NAA 2.0 mg/L medium was up to 364%, and the growth effect of embryogenic callus was the best when it was alternately cultured on the subculture medium with half the plant growth regulator.【Conclusion】Embryogenic callus
could be induced from‘Sanyuehong’and‘Yujinqiu.’The combination of inositol 100 mg/L+2,4-D 1.5 mg/L+KT 2.0 mg/L
was beneficial to embryogenic callus formation. The plant growth regulator combination of 2,4-D 1.5 mg/L+NAA 2.0 mg/L was
beneficial to the proliferation culture of embryogenic callus, and the growth effect of embryogenic callus was the best when it
was alternately cultured on the medium with half of the plant growth regulator and 2.0 mg/L AgNO3. |
