| 【Objective】The study was conducted to clarify the pathogen causing anthracnose disease of Coffea
arabica in Guagdong Province, and to screen out control fungicides.【Method】Tissue isolation method was used to isolate
and purify the pathogen, and pathogenicity test was conducted by using Koch’s postulates. The pathogen was identified
based on morphological characteristics and analysis of phylogenetic sequences（ITS, TUB2, GAPDH, CHS, ACT and GS）.
Moreover, the inhibitory effects of four common fungicides on the pathogen were measured according to mycelial growth.
【Result】The obtained strains were identified as Colletotrichum fructicola, C. siamense and C. musicola. The results of
pathogenicity assay indicated that all the selected isolates could infect leaves. In vitro test on two highly pathogenic strains
（C. fructicola CA-13 and C. siamense CA-16）showed that prochloraz, pyraclostrobin and thiophanate-methyl strongly
inhibited mycelium growth and their EC50 values were under 0.1 mg/L.【Conclusion】The dominant pathogen causing
anthracnose disease on C. arabica leaves were C. fructicola and C. siamense; prochloraz, pyraclostrobin and thiophanatemethyl could be used to control this disease as the first choice. |
