| 【Objective】The study was conducted to separate and prepare high-purity adenosine component and develop
a certified reference material（CRM）of adenosine.【Method】A method was established to separate and prepare a large amount
of high-purity adenosine from Trichosanthes kirilowii Maxim. fruit peel by macroporous resin chromatography combined with gel
column chromatography, preparative liquid chromatography. The structure of adenosine was identified and confirmed by means
of UV, IR, MS and NMR. The homogeneity and stability of adenosine samples were tested and passed the certification jointly
conducted by 8 laboratories according to the technical requirements of ISO Guide 35 and GB /T 15000. 3-2008.【Result】The
standard value was determined to be 99.89% and the uncertainty of confidence-level of 95% was 0.18%. The uniformity of
prepared samples well met the standard, and they were stable for 24 months under the storage condition of 2-8℃ , and accepted by
the expert group.【Conclusion】The preparation method of high-purity adenosine has the characteristics of low preparation cost,
simple operation and high efficiency, which solves the problem of the lack of national standard samples for adenosine, and provides
material basis to quality control, analysis and testing of T. kirilowii Maxim.medicinal materials and related products in China. |
