| 【Objective】Rabies virus (RABV) is a highly neurotropic virus, which composes of five structural proteins,
such as nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and RNA-dependent RNA polymerase (L).
The RABV genomic RNA, combining with N, P and L proteins, constitute the ribonucleoprotein complex (RNP). M proteins
plays a particular role in regulating the synthesis and assembly of the structural proteins of RABV. However, it is not clear
where the intracellular synthesis and translocation sites of M proteins are and whether there are differences in M proteins in
different cell lines among RABV strains. The co-localizations of M protein with ER and Golgi, M protein with G protein and
M protein with RNP in N2
A and BHK cells were studied to determine the intracellular synthesis pathway and translocation
of RABV M proteins.【Method】TCID50 of different RABV strains (CVS-11, SRV-9 and PB4) in N2
A or BHK cells were determined. Co-localization of M protein with ER and Golgi as well as with G protein and RNP (represented by N protein) in ER
and Golgi apparatuses were performed by Immunofluorescent.【Result】The titers in the different RABV strains had certain
differences. All the M proteins in different RABV strains were co-localized with ER and Golgi. M protein also co-localized with
G protein as well as with N protein; however, the co-localization of M protein and G protein was mainly in ER and Golgi, while
that of M protein and N protein in cytoplasm.【Conclusion】The synthesis and processing of the RABV M proteins were carried
out through ER-Golgi pathways. The study results confirmed the synthesis and translocation cellular sites of RABV M proteins,
and enriched the data for assembly and budding process of RABV virions. |
