| 【Objective】The effect of 6-BA on the flower forcing of butterfly orchid with a view to provide a basis for the regulation of flowering time of butterfly orchid, and to provide a reference for the flowering induction of varieties that are not easy to be induced.【Method】With‘Huofenghuang’butterfly orchid seedlings as test materials, by artificial forcing under the condition of air conditioning, single factor randomized block design was adopted and six 6-BA concentrations including 0（CK）, 5, 10, 15, 20, 25 and 30 mg/L were set up. The 6-BA was sprayed every 10 days, and the spraying
position for the butterfly orchid was the 2nd, 3rd and 4th blade section area（from top to bottom）. During the spraying period, the soluble sugar, soluble protein, CAT and POD activity of butterfly orchid leaf were determine, and the indexes such as pedicel extraction rate, double pedicel rate, pedicel length, days from treatment to pedicel extraction flower diameter and flower bud number were investigated.【Result】After being treated with 25 mg/L 6-BA for 30 d, the pedicel extraction, rate of butterfly orchid was 80.00%, while that of water control was only 11.76%. After being treated with 10-30 mg/L 6-BA for 40 d, the pedicel extraction rate of butterfly orchid was more than 90.00%, and all the pedicels were extracted after being treated for 50 d. The time from 25-30 mg/L 6-BA treatment to pedicel extraction was 29 days, which was significantly lower than that of water treatment（44 days）. In accordance with the morphological observation, the soluble protein content in the butterfly orchid leaves was the lowest and the soluble sugar content in the leaves was the highest before the flower buds were extracted. The CAT activity and POD activity of butterfly orchid leaf gradually decreased at the time of flower bud extraction.【Conclusion】The optimal concentration of 6-BA for butterfly orchid was 25 mg/L and the pedicle extraction rate reached the highest in a short period of time, which provided a reference basis for low temperature replacement. |
