| 【Objective】The study was carried out to isolate growth-promoting strains with high phosphorussolubilizing efficiency and explore the effects of different phosphorus-solubilizing bacteria strain stresses on the growth of Zea mays L. seedlings.【Method】The improved SRSM culture medium and Belimov method were used to screen out the phosphorus-solubilizing strains from the soil of winter wheat applied with chemical fertilizer in Pingliang City. The strains were identified by morphological characteristics and 16S rDNA sequence and their growth-promoting characteristics were analyzed. The resistance of Z. mays L. seedlings growth under different phosphorus bacteria stresses was studied by pot experiment.【Result】Among 8 preliminarily isolated phosphorus-solubilizing strains, the ZX-2020 strain was screened out, which was identified as Pseudomonas sp. through physiological and biochemical and 16S rRNA with the GenBank accession number was MT084758. The test results of Z. mays L. seedlings growth showed that, compared with those under different phosphorus-solubilizing strains treatments, the root length, stem length and leaf area of Z. mays L. seedling inoculated with ZX-2020 strain increased by 54.49%, 26.96% and 36.06%, respectively, with the IAA yield of 6.71 mg/ L and the dissolved phosphorus content of 198.26 μg/mL.【Conclusion】Pseudomonas sp. ZX-2020 can colonize better in contaminated soil and promote plant growth, which can be used as a good microbial resource for plant-microorganism in situremediation of chemical fertilizer contaminated soil. |
