| 【Objective】The moisture, ash and extract contents of Gynostemma pentaphyllum (GP) from different origins were studied, and the items and limits of quality control were set up with a view to provide references for the improvement of quality standard of GP. 【Method】The contents of moisture, total ash, acid-insoluble ash and water-soluble extracts of GP were determined according to Chinese Pharmacopoeia (2020 Edition).【Result】There were certain differences among the moisture, water-soluble extract, total ash and acid-insoluble ash of GP from different origins and different parts. Among all 75 batches of GP, the moisture content of the whole plant was the highest, which had significant difference compared with that of the three parts of tendrils, leaves and stem mixed with leaves. Among them, the moisture content of medicinal GP ranged from 8.31% to 12.78%; the total ash content ranged from 9.41% to 20.19%; the acid-insoluble ash content in 19 batches of samples (except for stems) ranged from 0.57% to 6.99%; and the water-soluble extract content was between 20.86% and 39.79%. Among 55 batches of tea GP, only 14 batches of moisture content and 19 batches of total ash content were qualified, the extract content of GP from Shaanxi, Hubei and Fujian wan higer than that from other provinces.【Conclusion】Combined with the comprehensive analysis on the quality control of medicinal and tea GP and experimental results, it is recommended that a unified standard should be developed. Four items of moisture, total ash, acid-insoluble ash and water-soluble extracts should be set as quality control inspection items of medicinal GP in each province, including: the moisture content of medicinal GP should not exceed 12.0%, the total ash content should not exceed 16.0%, the acid-insoluble ash content should not exceed 3.0%, and the water-soluble extract content should not be less than 25.0%; while quality control standards of tea GP can be developed based on the use of different parts. |
