【Objective】In order to explore the plant immune inducers which can be used to control tobacco bacterial
wilt, the antibacterial activity and control effects of four kinds of inducers were screened and determined.【Method】
The control effects were determined and analyzed by using plate inhibition method and potted plant experiments, and the
control effects of different plant immune inducers against tobacco bacterial wilt were studied through the determination of
minimum inhibition concentration(MIC)and minimum bactericidal concentration(MBC)value.【Result】Aminooligosaccharin and NANO ZnO showed a certain inhibitory effect on Ralstonia solanacearum in the plate tests. The 5 000
µg/mL amino-oligosaccharin had the best antibacterial effect with the inhibition zone diameter of 35.2 mm, and its MIC and
MBC were 1 000 µg/mL and 2 000 µg/mL, respectively. Different concentrations of Zhinengcong and Atarin did not show any
inhibitory effect. However, the potted plant experiments showed that Zhinengcong had the best prevention and control effect
on bacterial wilt. The disease entered the peak period on the 10th day after inoculation, and the control effect reached 57%, which was significantly higher than the 37% control effect of streptomycin treatment in the same period. Moreover, Zhinengcong
treatment could delay the occurrence of the disease. The control effect of amino-oligosaccharin with significant antibacterial
effect on the plate was only 13%, and the control effects of NANO ZnO and Atarin were 3% and 15%, respectively, with poor
control effects.【Conclusion】Plant immune inducer Zhinengcong had a great potential for biocontrol and can be used for green
prevention and control of tobacco bacterial wilt. |