| 【Objective】It has important guiding significance for perfecting rice seed quality inspection to carry
out TTC staining experiment, select the best combination and explore the changes in the main physiological indexes of
rice seeds.【Method】Single factor analysis and orthogonal experimental design were used to compare the influence of
seed imbibition time, TTC concentration, staining temperature and staining time, on the staining effect, and to screen
the best TCC staining combination. Spectrophotometry was used to determine the amylase activity and soluble sugar
content in rice embryos under different germination conditions.【Result】Rice embryos stained by TTC are divided
into five situations: The entire embryo was stained darkly; The entire embryo was stained lightly; The coleorhiza was
stained; The coleoptile was stained; The scutellum was stained. The influence of the four factors on the viability of rice seeds is as follows: staining time ＞ staining temperature ＞ TTC concentration ＞ imbibition time; The best condition for
TTC staining of rice seeds is as follows: staining time 2 h, staining temperature 30℃ , TTC concentration 1%, imbibition
time 3 h. Under different germination conditions, the changes of α-amylase, β-amylase activity and soluble sugars in
rice seeds were consistent with the changes in seed germination rate. 【Conclusion】Under the best combination of TTC
staining, the staining rate of rice seeds could accurately reflect the viability and germination ability of the seeds. Amylase
activity and soluble sugar content may be used as important physiological indicators for predicting and evaluating rice
seed vigor. |
