| 【Objective】The polysaccharides were extracted from the fruit body of Amauroderma rugosum, and the crude polysaccharides composition and anti-oxidant activity were analyzed, with a view to providing references for the development and utilization of Amauroderma rugosum resources.【Method】The crude polysaccharides from cultivated fruit body of A. rugosum were obtained by hot water extraction, deproteinization and freeze drying. HPLC, GPC and Kjeldahl method were used to analyze the components, molecular weight, protein and amino acid composition of polysaccharides, and the scavenging ability to hydroxyl radical in vitro, ABTS·+ free radical anti-oxidant activity were explored, the effect of polysaccharides on proliferation of macrophage Raw264.7 was also detected by MTS.【Result】The crude polysaccharides of A. rugosum was brown powder, and its scavenging ability to hydroxyl radicals and ABTS·+ radical were 90.26% and 83.95% respectively at the concentration of 1.5 mg/mL. It could also promote the proliferation of macrophages Raw264.7 in a certain range. The polysaccharide from the fruit body of A. rugosum was a protein-containing（16.99%）heteropolysaccharide, with 17 kinds of amino acid, of which 28% was essential amino acid, and the contents of Asp and Glu were the highest, accounting for more than 30%. The polysaccharides were composed of 10 monosaccharides, including galactose, glucose and mannose. The content of these three monosaccharides was 67.87%. The number-average molecular weight of the crude polysaccharide was 28.239 ku（Mn）, the weight-average molecular weight was 57.502 ku（Mw）and the distribution coefficient（Mw/Mn）was 2.036.【Conclusion】The crude polysaccharide from fruit body of A. rugosu is a heteropolysaccharide containing protein, which has good scavenging effect on hydroxyl radical and ABTS·+ free radical. The results of this study provide technical support for the promotion and scientific development of A. rugosum. |
