| 【Objective】Sweet-waxy maize is an important cash crop in South China, but the content of lysine
in its grain is low. The effects of opaque2 (o2) introduction on grain phenotype and lysine accumulation of sweet-waxy
maize were studied. 【Method】The o2 gene was introduced into sweet-waxy maize by conventional hybridization, the
polymorphism of donor and sweet-waxy maize receptor inbred lines was detected by SSR codominant markers closely
linked in o2 gene, and the backcross inbred offspring were detected by its specificity and codominant.【Result】Under
projection light, the seeds of donor parent HP3 were opaque, and the light transmittance of recipient material was good.
The hybrid panicle showed grain separation of different genotypes, hard and wrinkled phenotypes, and the grain light
transmittance of backcross offspring decreased significantly, which may be related to the existence of o2 gene. Through
the screening of three pairs of SSR markers umc1066, phi112 and phi057 of o2 gene, only marker phi057 showed good
polymorphism in donor and recipient inbred lines. Further study found that the o2 mutant gene of donor could be detected in backcross and inbred offspring, and successfully introduced into sweet-waxy maize receptor inbred lines.【Conclusion】
The early generation group system of o2 sweet / waxy maize was preliminarily created. The o2 gene plants of F1
and BC1
were
screened by SSR marker phi057, indicating that phi057 marker can effectively distinguish o2 gene plants. |
