| 【Objective】Dayemihe, a local famous and excellent tobacco variety in Fengkai County of Guangdong
Province, is an excellent source of resistance to bacterial wilt currently. In-depth study of the mechanism of tobacco
bacterial wilt resistance and search for differentially expressed proteins related to bacterial wilt resistance can provide
a reference for the in-depth study of the molecular mechanism of anti-bacterial wilt and disease resistance breeding.
【Method】With tobacco variety Dayemihe, resistant variety D101 and susceptible variety as materials, inoculation and identification of bacterial wilt were conducted indoor. Differentially expressed genes of Dayemihe before and after inoculation
were analyzed by Isobaric Tags for Relative and Absolute Quantitation（iTRAQ）and Quantitative Real-time PCR（qRTPCR）.【Result】After inoculation, the development of the bacteria wilt of Dayemihe was slower than that of the susceptible
control. Thirty-five proteins were differently expressed in Dayemihe: twenty-one proteins were down-regulated and fourteen
proteins were up-regulated. Among them, twenty-five proteins were related to plant stress. The protein with the up-regulated
differential expression with largest fold change was Hav1 belonging to the Nicotiana tabacum Agglutinin（Nictaba）family.
The nictaba within 12 hours after inoculation was detected by qRT-PCR. It was found that they had the highest expression
level at 6 hours and 9 hours after inoculation, and then the level began to decrease.【Conclusion】Plant response to
pathogen invasion is a complex process that requires proteins with different functions from different pathways. Plant response
to pathogen invasion is a complex process. According to the identification, the protein with the up-regulated differential
expression with largest fold change is Hav1, which belongs to the Nicotiana tabacum Agglutinin（Nictaba）family. Tobacco
lectins showed similar expression patterns after inoculation with highly pathogenic and non-pathogenic strains, indicating
that the lectin protein may play a role in the upstream reaction process after the invasion of pathogens. |
