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| A Preliminary Study on the Effects of Combination of Biocontrol Agents Against Watermelon Fusarium Wilt Caused by Fusarium oxysporum f. sp. niveum |
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| DOI：10.16768/j.issn.1004-874X.2022.01.011 |
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| Abstract: |
| 【Objective】The study was carried out to screen ef fective combinations of biocontrol agents against
wa termelon Fusarium wilt caused by Fusarium oxysporum f. sp. niveum【Method】Five biocontrol agents including
23（Bacillus amyloliquefaciens）, 25（Exiguobacterium acetylicum）, 28（Bacillus pumilus）, 37（Bacillus
licheniformis）and 156（Bacillus cereus）were combined to obtain 31 combinations. The plate bacteriostasis effects of
biocontrol agents and volatile organic compounds（VOCs）produced by each combination were studied via dual-culture
test and separation plate, then the effect of each combination on the germination rate of watermelon seeds and the growth
of seedlings as well as the biocontrol efficacy of these combinations against watermelon Fusarium wilt were determined.
【Result】The 31 combinations showed significant inhibition effect on FON growth, among which the inhibition rate of C28
was 68.06%, while the VOCs produced by C13（28+37）was 41.60%. There was no significant difference in the germination
rates of watermelon seeds pre-treated by various combinations and CK group, however, the gemination rate of seeds and
emergence rate of cotyledons presented a decreasing trended with the increase of the number of single isolates. In the pot
test, there were differences in the control effects of various combinations against watermelon Fusarium wilt, and the C22 had
the highest control efficacy of 61.1%.【Conclusion】There were differences in the plate antagonism and pot control effects
of 31 biocontrol combinations against watermelon Fusarium wilt, and it was predicted that the combination formed by two or
three single isolates had the best effect, and the antagonistic combinations are more beneficial to disease control. |
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