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| Metabolic Characteristics of Different Carbon Sources by Microorganisms in Sediment of High-density Tilapia Pond |
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| DOI：10.16768/j.issn.1004-874X.2022.01.015 |
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| Abstract: |
| 【Objective】Microbial community in sediment plays an important role in maintaining the balance of
pond ecosystem. The present research aimed to explore the characteristics and seasonal variation of carbon and nitrogen
metabolism of microorganisms in sediment of high-density tilapia pond.【Method】Sediment samples were collected
during the aquaculture season, and the contents of nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and total nitrogen
in sediment were analyzed. The average well color development (AWCD) of microbial metabolism of substrates containing
both C and N, containing both C and P and containing C only was analyzed by Biolog-ECO plates. 【Result】The contents of total nitrogen and ammonia nitrogen in pond sediment in July and October were significantly higher than those
in April. The microorganisms in the sediment could use substrates containing C only, including β-methyl-D-glucoside,
pyruvic acid methyl ester, D-galacturonic acid, tween 80, D-mannitol and D-cellobiose, substrates containing C and N,
including L-asparagine and N-acetyl-D-glucosamine (AWCD ＞ 0.5), and substrates containing C and P, including D,L-α-
glycerol phosphate and glucose-1-phosphate (AWCD ＜ 0.5). 2-hydroxy benzoic acid, itaconic acid and α-ketobutyric acid
could not be utilized. In April, AWCD of substrates containing C and N and substrates containing C only was higher
than those in July and October. AWCD of substrates containing C and N, and substrates containing C only was positively
correlated with nitrate content in sediment.【Conclusion】Biolog-ECO plate technology could be used to analyze nitrogen
use index of sediment microorganisms for evaluating nitrogen removal capacity of microbial community in sediment. |
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