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| Complete Genome Sequence Analysis of Guangdong Isolates of Cymbidium Mosaic Virus |
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| DOI：10.16768/j.issn.1004-874X.2022.02.013 |
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| Abstract: |
| 【Objective】Cymbidium mosaic virus (CymMV) is one of the most important orchid viruses. The wide
spread of CymMV poses a serious threat to the development of orchid industry. The study is conducted to explore the genetic
information and evolution of CymMV, which will provide an important scientific basis for monitoring and early warning of
orchid virus disease and genetic engineering of orchid virus disease in Guangdong Province. 【Method】RT-PCR and DASELISA were used to detect and identify CymMV from leaves with suspected virus disease of Cymbidium sinense collected
from Guangzhou area. The genome sequence assembly, annotation, phylogeny and selection p ressure analysis of CymMV
isolates were performed with related molecular biology software.【Result】Two CymMV isolates (GZV013 and ZC29) were
found in Guangdong Province for the first time in this study. Both of the genome lengths of GZV013 and ZC29 were 6 227 nt, encoding five functional proteins. The similarity analysis of the full sequence showed that the nucleotide sequence identity
of GZV013 and Taiwan isolate M2 was 97.03% and that of ZC29 and Nanjing isolate NJ-1 was 97.11%. The complete genome
sequence identity among CymMV isolates ranged from 86.85% to 98.31%, and the differentiation of diverse populations was
closely related to host species and geographical isolation. Each region of CymMV genome was affected by negative selection
and conformed to the neutral evolution model. The RdRp, TGB1 and TGB2 genes exhibited the highest genome-wide diversity.
【Conclusion】The GZV013 was most closely related to Taiwan isolate M2, and ZC29 was most closely related to Nanjing
isolate NJ-1, belonging to the same branch of a family. |
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