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| Effect of Circular RNA hsa_circ_004389 onReplication of Influenza A Virus |
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| DOI：10.16768/j.issn.1004-874X.2022.02.016 |
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| Abstract: |
| 【Objective】Based on the preliminary transcriptome profiles of A549 cells responding to influenza A virus
(IAV) infection, one of the most significantly up-regulated host circular RNA(circRNA), hsa_circ_004389, was selected to
further validate its expression and explore its roles in IAV replication.【Method】First, real-time quantitative PCR (qPCR)
was used to verify the expression levels of hsa_circ_ 004389 at different time courses and different multiplicity of infection
(MOI) of IAV infection in A549 cells; Second, hsa_circ_ 004389 expression in A549 cells treated with poly: I∶C (polyinosinic
acid ∶ polycytidylic acid) and LPS (Lipopolysaccharide) were detected, respectively; Third, A549 cells were treated with
interferon beta (IFNβ) and JAK inhibitor, and the expression of hsa_circ_004389 was determined; Finally, after a transient
overexpression and knockdown in A549 cells, IAV replication at mRNA and protein levels were determined.【Result】
There was a positive correlation between the expression of hsa_circ_004389 and IAV replication. JAK-STAT signaling path
was involved in regulating hsa_circ_004389 expression, and hsa_circ_004389 had the ability to inhibit IAV replication. 【Conclusion】Hsa_circ_004389, the product of the host responding to IAV infection, is a potential host anti-IAV factor. |
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