|
| Construction of Genetic Linkage Map of Plum (Prunus salicina L.) with ISSR and SRAP Markers |
| |
| DOI：10.16768/j.issn.1004-874X.2022.03.005 |
|
| Hits: 1020 |
| Download times: 664 |
| Abstract: |
| 【Objective】Plum (Prunus salicina L.) is a tree species with great economic prospect in northeast China,
and it has important social and economic values. Genetic linkage maps of plum were constructed by molecular markers to
provide a theoretical basis for breeding new plum varieties.【Method】Jilin 6 and Longyuan Qiuli were used as the mapping
parents. The authenticity of F1
progeny was identified by SSR primers. 14 ISSR and 16 SRAP primers were selected to
construct the genetic linkage map of plum by using Join Map 4.0 software and “double pseudo-testcross” theory.【Result】
In this study, 72 authentic F1
progeny were obtained. ISSR and SRAP primers produced 5 segregation patterns and 120
marker loci in this F1
population. A genetic linkage map with 16 linkage groups and 38 marker loci with a total length of
528.5 cM was constructed by software analysis. The groups ranged in size from 9.6-71.1 cM, and the average size was 13.9
cM. The marker had a maximum genetic distance of 52.8 cM and a minimum genetic distance of 1.4 cM.【Conclusion】The
construction of genetic linkage maps enriched the genetic research basis of plum in cold region, and contributed to the study
of QTL mapping, marker-assisted breeding and gene mapping. |
|
View Full Text
View/Add Comment Download reader |
|
|
|
