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| Establishment of Tissue Culture and Rapid PropagationSystem for Paris polyphylla var. chinensis |
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| DOI：10.16768/j.issn.1004-874X.2022.05.002 |
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| Abstract: |
| 【Objective】The study was conducted to establish a tissue culture and rapid propagation system for
Paris polyphylla var. chinensis.【Method】The seedlings of P. polyphylla var. chinensis were selected as materials
in this experiment. Different pretreatment and sterilization method for explants were studied. And the optimum
explants, the suitable medium for adventitious buds induction, propagation, rooting and bulb setting were also screened.
【Result】The contamination rate reached the lowest (1.67%) and the new buds grew best after the explants was dipped
into 0.5% carbendazim for 2 h and then dipped into 75% ethanol for 45 s, and 0.1% HgCl2
for 10 min. The best medium
for adventitious buds induction was WPM+2.0 mg/L ZT+0.1 mg/L NAA+30 g/L sucrose, the induction rate reached up to 98.33% and new buds grew fast and well when the seedlings or the seedlings without leaves and stems were selected as explants,
which were 5-10 cm high with roots. The optimal medium for adventitious buds propagation was MS + 2.0 mg/L 6-BA+1.0
mg/L IAA+30 g/L sucrose and the propagation coefficient reached 3.75 times after being cultured for 90 d. While the optimal
medium for adventitious buds rooting and bulb culture was WPM+2.0 mg/L ZT+0.1 mg/L NAA+30 g/L sucrose, the rooting and
bulb setting rate reached 96.67%, the root number was 3.84, the root length was 4.15 cm and the bulb diameter was 0.86 cm.
The best medium for transplanting and domestication of seedlings was peat soil ∶ perlite ∶ river sand = 2 ∶ 1 ∶ 1 on March
to April and the survival rate reached 30%.【Conclusion】A tissue culture and rapid propagation system for P. polyphylla
var. chinensis seedlings was established, which laid a foundation for resource conservation and mass production of seedlings of
P. polyphylla var. chinensis. |
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