|
| Comparative Genomics Analysis ofProtease Genes in Bacillus sp. |
| |
| DOI：10.16768/j.issn.1004-874X.2022.05.013 |
|
| Hits: 976 |
| Download times: 743 |
| Abstract: |
| 【Objective】The study aims to explore the relationship between the protease-producing capacity of
Bacillus and the protease genes carried by the bacteria and analyze the important enzyme genes that affect the proteaseproducing capacity of Bacillus.【Method】The protease-producing strain was screened by using the selective medium.
The protease activity of the strains was determined by the Folin method. The protease genes carried on the chromosomes of
the strains were obtained and classified by sequencing the whole genome of 15 strains of Bacillus. The transcription level of
protease gene mRNA was measured by the real-time fluorescent quantitative PCR method. Through comparative genomics and Bacterial Genome-Wide Association Analysis (BGWAS), the reasons that affect the ability of bacteria to produce protease
were explored. 【Result】According to the protease genes carried on the chromosomes of the strains, they could be divided
into 7 groups of genotypes (G1-G7). Comparative genomics and BGWAS found that the number of protease genes carried on
bacterial chromosomes was directly related to the protease production capacity, and the protease production capacity of strains
lacking aprX and aprE was significantly reduced. The difference in the protease production capacity of Bacillus was found to
be related to the expression level of aprX by further studying the mRNA level of the gene.【Conclusion】There is a correlation
between the protease gene carried on the chromosome of the strain and the protease production capacity of the strain. The
important enzyme gene that affects the protease production capacity of Bacillus is aprX. |
|
View Full Text
View/Add Comment Download reader |
|
|
|
