| 【Objective】The study was carried out to develop new molecular markers and analyze the molecular
phylogeny of Magnolia officinalis in Longshan Tree Farm, Lechang, Guangdong, with a view to facilitating subsequent
researches on the selection of excellent plants and breeding of superior varieties.【Method】Fourteen Magnolia offi cinalis
(provenances from Jiangxi Province) individual plants collected from Longshan Tree Farm were used as tested materials. By
using Primer BLAST on NCBI (https://www.ncbi.nlm.nih.gov), fixed primers of TRAP molecular markers were designed based
on the sequences of M. offi cinalis cDNA in the GenBank. The fixed primers were matched with random primers (sequence
related amplified polymorphism) to amplify genomic DNA and develop TRAP molecular markers. The electrophoretogram of
TRAP markers and amplified products of M. offi cinalis SSR (Simple Sequence Repeat) were counted. Then, NTSYSpc2.10e
was used for cluster analysis to discover the genetic diversity and phylogeny of fourteen M. offi cinalis resources.【Result】
Seven TRAP molecular markers showing good polymorphism and clear electrophoresis bands were successfully developed
from seventy primer pairs formed by fourteen fixed primers and five random primers. The phylogenetic tree of M. offi cinalis plants was constructed. The genetic similarity coefficient of these M.offi cinalis plants was 0.75-0.88 and it was not the highest
between mother plant and its offspring.【Conclusion】M. officinalis was rich in genetic diversity and gene exchange was
frequent in M. offi cinalis population. The newly developed TRAP molecular markers will facilitate identification of superior
plants, breeding of excellent varieties and marker-assisted selection of M. offi cinalis in the future. |
