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| Isolation, Identificat ion and Biological Characteristicsof A Strain of Purpureocillium lilacinum |
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| DOI：10.16768/j.issn.1004-874X.2022.06.014 |
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| Abstract: |
| 【Objective】The study was conducted to investigate the growth, protease and chitinase activities
of a strain of Purpureocillium lilacinum isolated from the stroma of Aschersonia aleyrodis on citrus under different
culture conditions, with a view to providing a theoretical basis for the use of P. lilacinum as a biocontrol agent.
【Method】The strains of the stroma of A. aleyrodis from Hongjiang orange leaves were isolated and purified by tissue
isolation, identified by a combination of morphological characteristics and molecular methods, and their protease and
chitinase activities were determined by the Folin-phenol and DNS method .【Result】The strain ZJPL1812 was identified as
P. lilacinum by morphological observation and ITS sequence analysis. The best culture temperature for the strain was 30℃ ,
and the suitable pH value was 5.0-9.0. The best mycelial growth was achieved when ammonium dihydrogen phosphate and
maltose were used as the nitrogen and carbon sources; the highest protease activity was produced when peptone and sucrose
were used as the nitrogen and carbon sources; and the highest chitinase activity was achieved when ammonium nitrate
and soluble starch were used as the nitrogen and carbon sources.【Conclusion】The strain can use many different kinds
of nitrogen and carbon sources, with a wide range of acid-base adaptability and strong environmental adaptability. And it
produces high protease and chitinase activities, indicating that it has some potential in biological control. |
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